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      常用培養基配方一

      發布時間:

      2022-12-24

      作者:

      表面接觸培養皿生產公司


      1. Acetobacter Medium[醋酸菌培養基]
      Glucose[葡萄糖] 100g,Yeasst extract[酵母膏] 10g,CaCO3 20g,Agar[瓊脂] 15g,Distilled water[蒸餾水] 1000毫升,Adjust[調] pH to 6.8
      適用范圍:惡臭醋酸桿菌混濁變種。
       
      2. Nutrient Agar[營養肉汁瓊脂]
      Pepton[蛋白胨] 5g Beef extract[牛肉膏] 30g,NaCl 5g Agar[瓊脂] 15g,Distilled water[蒸餾水] ,Adjust[調] ,pH to 7.0-7.2
      [Note]:When cultivation of Bacillus,5mg of to MnSO4.H2O may be added .
      It is favorable to promote spore formation .
      適用范圍:產氣氣桿菌、糞產堿桿菌、蠟狀芽孢桿菌、蠟狀芽孢桿菌蕈狀變種、地衣形芽孢桿菌、巨大芽孢桿菌、多粘芽孢桿菌、塵埃芽孢桿菌、短小芽孢桿菌、嗜熱脂肪芽孢桿菌、枯草芽孢桿菌、枯草芽孢桿菌深黑變種、蘇云金芽孢桿菌、蘇云金芽孢桿菌蠟螟亞種[青蟲菌]、蘇云金芽孢桿菌戈爾斯德變種、蘇云金芽孢桿菌猝倒亞種、產氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、大腸埃希氏菌[大腸桿菌]、銅綠假單胞菌[綠膿桿菌]、凸形假單胞桿菌、熒光假單胞菌、彎曲假單胞菌、惡臭假單胞菌、假單胞桿菌、藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運動發酵單孢菌
       
      3. Azotobacter Medium[固氮菌培養基]
      KH2PO4 0.2g K2HPO4 0.8g,MgSO4.7H2O 0.2g CaSO4.2H2O 0.1g,Na2MoO4.2H2O Trace[微量] Yeast axtract[酵母膏] 0.5g,Mannitol[甘露醇] 20g FeCl3 Tract[微量],Distilled water[蒸餾水] 1000毫升 Agar[瓊脂] 15g,Adjust[調] pH to 7.2
      適用范圍:固氮菌、膠質芽孢桿菌。
       
      4. Corn Meal Medium[玉米粉培養基]
      Maize flour[玉米粉] 5g,Peptone[蛋白胨] 0.1g,Glucose[葡萄糖] 1g,Tap water[自來水] 1000毫升
      [Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 毫升 of the liquid , then autoclave at 121℃ for 1 hr . again[15磅蒸煮1小時,分裝入18ⅹ18毫米試管,每管深度達6厘米。15磅再次滅菌15小時。]
       
      5. Lactic-bacteria Medium I[乳酸菌培養基 I ]
      Yeast extract[酵母膏] 7.5g,Peptone[蛋白胨] 7.5g,Glucose[葡萄糖] 10g KH2PO4 2g,Tomato juice[西紅柿汁] 100毫升 Tween[吐溫] 80 0.5毫升,Distilled water[蒸餾水] 900毫升 pH 7.0
      適用范圍:植物乳桿菌[胚芽乳桿菌]、嗜熱乳酸鏈球菌。
       
      6. Lactic-bacteria Midium Ⅱ[乳酸菌培養基 Ⅱ]
      Lacto-casein peptone[乳酪蛋白胨] 10g,Beef extract[蛋白胨] 10g,Yeast extract[酵母膏] 5g Glucose[葡萄糖] 5g,Tween[吐溫] 80 1g K2HPO4 2g,Na-acetate[醋酸鈉] 5g,Diamine citrate[檸檬酸二胺] 2g,MgSO4.7H2O 0.2g,MnSO4.H2O 0.05g
      Distilled water[蒸餾水] 1000m pH 6.5-6.8
      適用范圍:植物乳桿菌[胚芽乳桿菌]。
       
      7. Peotone Glucose Yeast extract Medium PGY [蛋白胨、酵母膏、葡萄糖培養基]
      Peptone[蛋白胨] 10g,Yeast extract [酵母膏] 5g,Glucose [葡萄糖] 1g,Distilled water [蒸餾水] 1升
       
      8. Glycerol Agar [甘油瓊脂]
      Peptone [蛋白胨] 5g,Beef extract [酵母膏] 3g,Glycerol [甘油] 20g,Top water [自來水] 1000毫升,Agar [瓊脂] 15g,pH 7.0-7.2
       
      9. Rhizobium medium [根瘤菌培養基]AS 9
      Yeast eztract [酵母膏] 1g,Soil eztract [土壤浸提液] 200毫升,Mannitol [甘露醇] 10g,Agar [瓊脂] 15g,Distilled water [蒸餾水] 800毫升,pH 7.2
      [Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200毫升 of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200毫升. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed.[土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小時,經濾紙過濾后加水補足到200毫升。]
      適用范圍:大豆根瘤菌[慢生型]、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、大豆根瘤菌[快生型]、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌。
       
      10. Mannitol Agar[甘露醇瓊脂]
      Yeast extract[酵母膏] 5g,Peptone[蛋白胨] 3g,Mannitol[甘露醇] 25g Agar[瓊脂] 15g,Distilled water[蒸餾水] 1000毫升
       
      11. Glucose Asparagine [葡萄糖、天門冬素瓊脂]
      Glucose [葡萄糖] 10g,Asparagine [天門冬素] 0.5g,K2HPO4 0.5g,Water [水] 1000毫升,pH 7.2-7.4
      適用范圍:刺孢小單孢菌絳紅變種、紫色小單孢菌[絳紅小單孢菌]。
       
      12. Gause′s Synthetic Agar [高氏合成一號瓊脂]
      KNO3 1g Soluble starch[可溶性淀粉] 20g,K2HPO4 0.5g,MgSO4.7H2O 0.5g,NaCl 0.5g,FeSO4 0.01g,Agar [瓊脂] 20g,water [水] 1000毫升,pH 7.2-7.4
      適用范圍:刺孢小單孢菌絳紅變種、紫色小單孢菌[絳紅小單孢菌]、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產色鏈霉菌、燼灰鏈霉菌、天藍色鏈霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球孢鏈霉菌、淺灰鏈霉菌、灰色鏈霉菌、吸水鏈霉菌、淡紫灰鏈霉菌、黃色長孢鏈霉菌、藤黃色鏈霉菌、細黃鏈霉菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內瑞拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌。
       
      13. Wort Agar [麥芽汁瓊脂]
      Dilute the world[without hop] to 12 Brix. Add 15g agar into 1000毫升 of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes.[將發酵啤酒的原料[未加酒花],稀釋至12柏林,加瓊脂15克,溶化后分裝。15磅滅菌30分鐘。]
      適用范圍:克魯斯假絲酵母、郎比可假絲酵母、解脂假絲酵母、馬其頓假絲酵母、擬熱帶假絲酵母、粗壯假絲酵母、皺褶假絲酵母、熱帶假絲酵母、產朊假絲酵母、阿舒假囊酵母、白地霉、果香地霉、地霉屬、異常漢遜酵母、異常漢遜酵母變種、阿拉伯糖醇漢遜酵母、施氏漢遜酵母、菅囊酵母、伯頓畢赤酵母、膜醭畢赤酵母、粘紅酵母、小紅酵母、膠紅酵母、深紅酵母、卡爾斯伯酵母、釀酒酵母、椰園釀酒酵母、發酵性酵母、洛格酵母、羅斯酵母、魯氏酵母多形魯氏酵母、威爾酵母、酵母、路德類酵母、栗酒裂殖酵母、擲抱酵母、貝雷絲孢酵母、皮狀絲孢酵母、糙孢曲霉、無殼曲霉、鮮橙曲霉、紅曲霉、紫紅曲霉、紅色紅曲霉、紅曲霉菌
       
      14. Potato Dextrose Agar PDA[馬鈴薯、葡萄糖瓊脂]
      Potato extract[馬鈴薯汁] 1000毫升,Dextrose[glucose][葡萄糖] 20g,Agar[瓊脂] 20g
      [Note]: Potato extract: Slice potatoes thin. Add 1000毫升 of water, immediately to prevent Oxidtion of juice and boil until soft.[approximately 30min.]. Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.[注:取去皮馬鈴薯200克,切成小塊,加水1000毫升煮沸30分鐘,濾去馬鈴薯塊,將濾液補足至1000毫升,加葡萄糖20克,瓊脂15克,溶化后分裝,15磅滅菌30分鐘。]
      適用范圍:酵米面假單胞菌[酵米面黃桿菌]、白色鏈霉菌、燼灰鏈霉菌、青色鏈霉菌、球孢鏈霉菌、灰色鏈霉菌、龜裂鏈霉菌、傘枝梨頭霉、雅致放射毛霉、棒曲霉、米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛殼、長刺毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、束狀刺盤孢、新月彎孢霉、奇異翅孢殼、地生翅孢殼、串珠鐮孢、尖鐮孢、盤長孢菌[魯保一號]、銀白楊盤長孢、玉蜀黍長蠕孢、深黃被孢霉、小被孢霉、多頭被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、擬青霉、球形阜孢、白腐菌、少根根、華根霉、科恩根霉、戴爾根霉、日本根霉、爪哇根霉、米根霉、點頭根霉、綠穂霉、簇孢匍柄霉、雅致枝霉、康寧木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢。
       
      15. Czapek′s Agar[察氏瓊脂]
      Sucrose [蔗糖] 30g,NaNO3 3g,MgSO4.7H2O 0.5g KCl 0.5g,FeSO4.7H2O 0.01g,K2HPO4 1g,Agar[瓊脂] 13g,Distilled water[蒸餾水] 1000毫升,pH 7.2
      適用范圍:紅霉素鏈霉菌、洋蔥曲霉、金頭曲霉、泡盛曲霉、泡盛曲霉煙色變種、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、無花果曲霉、黃柄曲霉、黃曲霉、煙曲霉、構巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、醬油曲霉、土曲霉、宇佐美曲霉、焦曲霉、雜色曲霉、溫特曲霉、阿達青霉、產黃青霉、黃綠青霉、桔青霉、頂青霉、園弧青霉、繩狀青霉、灰黃青霉、島青霉、淡紫青霉、特異青霉、赭綠青霉、產紫青霉、婁地青霉、小刺青霉、蕁麻青霉、
       
      16. Concentrated Sucrose Czapek′s[濃糖察氏瓊脂]
      Sucrose[蔗糖] 200g,NaNO3 3g,MgSO4.7H2O 0.5g,KCl 0.5g,FeSO4.4H2O 0.01g,K2HPO4 1g,Agar[瓊脂] 13g,Distilled water[蒸餾水] 1000毫升
      適用范圍:謝瓦曲霉
       
      17. Synthetic Potato Medium[綜合馬鈴薯培養基]
      20%Potato extract[馬鈴薯汁] 1升,Glucose[葡萄糖] 20g,KH2PO4 3g,MgSO4.7H2O 1.5g,Vitanib B1[硫胺素] Trace[微量] Agar[瓊脂] 15g,pH 6
      適用范圍:米曲霉、醬油曲霉、棲土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛客、長刺毛殼、 球毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、多主枝孢、束狀刺盤孢、葫蘆科刺盤孢、新月彎孢霉、奇異翅孢殼、地生翅孢殼、串珠鐮孢、蝕脈鐮孢霉、盤長孢菌[魯保一號]、銀白楊盤長孢、瓶毛殼、金龜子綠僵菌、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、淡紫色擬青霉、擬青霉、白腐菌、少根根霉、根霉屬的菌、華根霉、科恩根霉、戴爾根霉、臺灣根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、點頭根霉、三寶壟根霉、根霉屬的種、綠穂霉、康寧木霉、木素木霉、擬康氏木霉、木霉、粉紅木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢、雙孢蘑菇、大肥菇、發光假蜜環菌、木耳、皺木耳、毛木耳、大禿馬勃、長根菇、金針菇、棘托竹蓀、短裙竹蓀、短裙竹蓀東北變種[暫定]、長裙竹蓀、白鬼筆、無裙竹蓀、寬鱗大孔菌、凸圓靈芝[白芝]、紫芝、靈芝[紅芝,赤芝,日本靈芝,韓國靈芝]、灰樹花、猴頭菌、香菇、粗毛斗菇、潔麗香菇、豹皮菇、玉蕈、真姬菇、安絡小皮傘菌、鬼毛針、黑柄皮傘菌、尖頂羊肚菌、圓錐羊肚菌、高羊肚菌、黑脈羊肚菌、羊肚菌、滑菇、光帽黃傘、尖鱗黃傘、金頂側耳、榆黃磨、金頂磨、白黃側耳、紫孢平菇、姬菇、裂皮側耳、擬囊體側耳[鮑魚菇]、真線側耳短孢變種、真線側耳、扇形平菇、扁形平菇、佛羅里達無孢子側耳、佛羅里達側耳、蠔菇、糙皮側耳、平菇、貝形側耳、鷹翅菌、肺形側耳、柳樹菌、樹窩、扁柄側耳、美味側耳、紫孢側耳、粉褶側耳、粉紅側耳、鳳尾菇、漏斗狀側耳、榆干側耳、大榆磨、側耳、茯苓、黑菇、煙色紅菇、裂褶菌、雞縱、金耳、黃木耳、銀耳、白木耳、紫晶口磨、銀絲菇、絲蓋小包腳菇、草菇。
       
      18. Corn Meal Medium[玉米粉培養基]
      Mix corn meal with water [20:100[W/V]] well . Distribute the mixture into 100毫升 bottles , each bottle contains about 30毫升 of the mixture . Autoclave at 121℃ for 30minutes .[20%玉米粉裝瓶,15磅滅菌30分鐘。]
       
      19. Filter Paper Medium [濾紙培養基]
      [NH4]2SO4 1g,KH2PO4 1g,MgSO4.7H2O 0.7g,NaCl 0.5g,Distilled water [蒸餾水] 1000毫升,A strip of filter paper [濾紙條] 6ⅹ1cm [厘米]
       
      20. Soybean Cake Meal Agar[黃豆餅粉瓊脂]
      10% infusion of soybean cake meal[10%黃豆餅粉浸汁] 1000毫升,Glucose[葡萄糖] 10g,NaCl 2.5g
       
      21. Sawdust, Wheat-bran Medium [木屑、麩皮培養基]
      Sawdust of broad leaf tree 75g,Wheat bran 25g
      Preparation:Mix these two ingredients well . Add appropriate water and blend[the amount of water added may be becided by kneading with fingers ]. Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth .
      殼斗科植物或闊葉樹的木屑 75克,麩皮 25克
      制法:加水適量充分混勻,水量以手捏時指間有水珠為宜,裝瓶并壓緊,在中心處扎一小洞,堵上棉塞,20磅滅菌1小時。
       
      22. Pine Block or Pine Sowdust Medium [松木條或松木屑培養基]
      1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr .[將10ⅹ2ⅹ1厘米的松木條浸泡在1-2%的蔗糖水中。使木條吸足糖水,裝瓶不宜過緊,用紙包好,20磅滅菌1小時。]
      2. Pine Sawdust Medium
      Pine Sawdust[松木屑] 75%,Rice bran[米糠] 23-24%,Socrose[蔗糖] 1-2%,Water[appropriate]水 [適量]
      Autoclave at 20 lbs for 1 hr.[20磅滅菌1小時]
       
      23. Rice Straw. Dung Medium[稻草、馬糞培養基]
      Dried horse dung[or cow dung] [干馬糞或牛糞] 65%,Died straw [干稻草] 35% Urea [尿素] 0.5%,Calcium superhosphate [過磷酸鈣]0.7% Gypsum powder[石膏粉] 1%,Ammonium sulphate [硫酸銨] 0.3%
      [Note]: Add appropriate water, pile them up and cause them fermentation. [水適量進行堆積發酵]
       
      24. Straw Rice Bran Medium [稻草、米糠培養基]
      Dried straw [干稻草] 75-80%,Rice bran [米糠] 20-25%
      [Note]:Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully[the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers].[ [注]:選無霉稻草,切成長約3厘米的小段,用清水浸泡12小時,使充分吸水,然后傾去水,加米糠和水,水量以用手捏時指間有水珠為宜。]
       
      25. Rice Medium[米飯培養基]
      Fill 10g of rice into a 50毫升 of Erlenmeyer flask. Add 20-30毫升 water. Autoclave at 121℃ for 30 minutes[or fill 2g of rice into a 15ⅹ150mm tube]. Add 6 毫升 of water. Autoclave at 121℃ for 30 minutes.[取大米10克,入50毫升椎形瓶中,水20-30毫升于15磅滅菌30分鐘即成,或取大米2克,裝入15ⅹ150毫米試管中,加水6毫升,于15磅滅菌30分鐘。]
       
      26. Filter Paper Medium[濾紙條培養基]
      [NH4]2SO4 1g,KH2PO4 1g,MgSO4.7H2O 0.5g,Yeast Extract[酵母膏] 0.1g,Distilled Water[蒸餾水] 1000毫升,Strip of filter paper 1ⅹ7 cm[濾紙條 1ⅹ7 厘米]
      [Note]:Siddolve the above ingredients. Distribute the liqid medium into tubes[each tube contains 1毫升 of the broth]. Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically.[將上述溶液分配成溶液后分裝入試管,每管1毫升,15磅滅菌30分鐘。再將滅菌的濾紙條[1條或2條]投入試管中即好使用。]
       
      27. Yeast Extract-Medium Extract Agar I[酵母膏、麥芽膏瓊脂]
      Yeast extract[酵母膏] 10g,Mall extract[麥芽膏] 10g,Glucose[葡萄糖] 4g,Distilled water[蒸餾水] 1000毫升,Agar[瓊脂] 20g,pH 7.0
      適用范圍:紅霉素鏈霉菌。
       
      28. Yeast Extract Peptone [酵母膏、蛋白胨瓊脂]
      Yeast extract [酵母膏] 1g,Multi-peptone[多蛋白胨] 2g,Beef extract [牛肉膏] 1g,Glucose [葡萄糖] 10g,Agar [瓊脂] 20g,Distilled water [蒸餾水] 1升,pH 7.0
       
      29. Glucose Yeast Extract Starch Agar [葡萄糖、酵母、淀粉瓊脂]
      Glucose [葡萄糖] 10g,Yeast extract [酵母膏] 10g,Starch [淀粉] 10g,NaCl 5g,CaCO3 3g,Agar [瓊脂] 20g,Distilled water [蒸餾水] 1升 pH 7.0
       
      30. Wheat Bran Medium [麩皮培養基]
      Wheat bran [麩皮] 36g,[NH4]2HPO4 10g,K2HPO4 0.2g,MgSO4。7H2O 0.1g,Agar [瓊脂] 20g,Distilled water [蒸餾水] 1升,pH 7.0
       
      31. Yeast Sporulation Medium[酵母菌產生子囊孢子培養基]
      [1] Kleyn Medium
      KH2PO4 0.012%,K2HPO4 0.02%,Sodium acetate[醋酸鈉] 0.5%,Glucose[葡萄糖] 0.062%,NaCl 0.062%,Peptone[蛋白胨] 0.25%,Biotin[生物素] 2μg%,Agar[瓊脂] 2%
      Trace element solution[混合鹽類] 1毫升/100毫升
      [Note]: Trace element solution [混合鹽類溶液]: MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
      [2] Gorodkowa Medium
      Glucose[葡萄糖] 0.1%,Peptone[蛋白胨] 1%,NaCl 0.5%,Agar[瓊脂] 2%
      [3] McClary Medium
      Glucose[葡萄糖] 0.1%,KCl 0.18%,Yeast juice[酵母汁] 0.25%,Sodium actate[醋酸鈉] 0.82%,Agar[瓊脂] 1.5%
       
      32. BPY Medium[BPY 培養基]
      Beef extract[牛肉膏] 5g,Prptone[蛋白胨] 10g,NaCl 5g Yeast extract[酵母膏] 5g,Glucose[葡萄糖] 5g,Agar[瓊脂] 1-2%,Distilled[蒸餾水] 1000毫升,pH 7.0
      適用范圍:大腸埃希氏菌[大腸桿菌]、惡臭假單胞菌。
       
      33. LB Medium[LB 培養基]
      Yeast extact[酵母膏] 5g,Peptone[蛋白胨] 10g,NaCl 10g,Agar[瓊脂] 1-2%,Distilled water[蒸餾水] 1000毫升,pH 7.0
      適用范圍:大腸埃希氏菌[大腸桿菌]。
       
      34. Penssay Broth[Penssay 肉湯]
      Beef extract[牛肉膏] 1.5g,Yeast extract[酵母膏] 1.5g,Tryptone[胰蛋白胨] 5g NaCl 3.5g,Glucose[葡萄糖] 1g,K2HPO4.3H2O 4.8g,Distilled water[蒸餾水] 1000毫升,pH 7.2
       
      35. Blond Agar[血瓊脂培養基]
      Peptone[蛋白胨] 10g,Glucose[葡萄糖] 2g,NaCl 5g NaH2PO4 2.5g,Sodium citrate[檸檬酸鈉] 5g,Brain infusion[腦浸汁] 800毫升,Pig heart infusion[豬心浸] 200毫升,Sleep Blood[羊血] 10%,pH 7.2
      [Note]:Preparation of pig brain and pig heart infusion:
      [1] Smash up 100g fresh pig heart [without fat and blood vessel].Add distilled water up to 1000毫升;[2] Cut 200g fresh pig brain[without blood vessel]. Add distilled water up to 1000毫升;[3] Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;[4] Cool and filtrate through gauze . [注]豬心和豬腦浸汁法:[1]新鮮豬心除去心頭和脂肪,攪碎100克加蒸餾水至1000毫升;[2]新鮮豬腦除去血管,取200克加蒸餾水至1000毫升;[3]50℃保溫1小時,然后煮開15分鐘;[4]冷卻后用紗布過濾備用。
       
      36. Cellulose﹣decomposing Bacteria Synthetic Medium[纖維細菌合成培養基]
      NaCl 6g MgSO4.7H2O 0.1g,KH2PO4 0.5g CaCl2 0.1g,K2HPO4 2.0g,Yeast extract[酵母膏] 1.0g,[NH4]2SO4 2.0g,Distilled water[蒸餾水] 1000毫升,Filter paper strip: one strip[濾紙條:一片],pH 7.0-7.4
      [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. [斜面培養基去濾紙條用2%瓊脂及2%羧甲基纖維素鈉 CMCNa ]
       
      37. Clostridium Pasreurianum Synthetic Medium [巴氏梭狀芽孢桿菌合成培養基]
      Glucose [葡萄糖] 1%,MnSO4 0.001%,KH2PO4 0.05%,FeSO4 0.001%,K2HPO4 0.05%,Yeast extract[酵母膏] 0.1%,MgSO4.7H2O 0.02%,Peptone[蛋白胨] 0.01%,NaCl 0.001%,CaCO3 0.5%,Distille water[蒸餾水] 100% pH 7.0
       
      38. Yeast Extract-Medium Extract Agar Ⅱ[酵母膏、麥芽膏瓊脂 Ⅱ]
      Yeast extract [酵母膏] 4g,Mall extract [麥芽膏] 10g,Glucose [葡萄糖] 4g,Agar [瓊脂] 17g,Distilled water [蒸餾] 1000毫升,pH 7.3
       
      39. Oatemeal Agar [燕麥粉瓊脂]
      Oatemeal[infusion][燕麥粉浸液] 20g,Agar [瓊脂] 18%,Trace element solution [跡量鹽溶液]1毫升,Distilled water up to [用蒸餾水補充至1000毫升] 1000毫升,pH 7.2
      [Note]:Trace element solution[跡量鹽溶液]:
      FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g,ZnSO4.7H2O 0.1g,Distilled water[蒸餾水] 100毫升
       
      40. Calcium Malate Agar[蘋果酸鈣瓊脂]
      Calium malate[蘋果酸鈣] 10g,K2HPO4 0.5g,NH4Cl 0.5g,Glycerol[甘油] 10g,Agar[瓊脂] 15g,Distilled water[蒸餾水] 1000毫升
       
      41. Thiobacillus Ferrooxidans Medium[氧化亞鐵硫桿菌培養基]
      [NH4]2SO4 0.15g,KH2PO4 0.05g,KCl 0.05g,MgSO4.7H2O 0.5g,Ca[NO3]2.4H2O 0.01g,FeSO4.7H2O 50g,Distilled water[蒸餾水] 1000毫升,pH 2.0
       
      42. Thiobacillus Thiooxidans Medium[氧化硫硫桿菌培養基]
      [NH4]2SO4 0.3g,KH2PO4 3-4g,CaCl2[anhydrous] [無水] 0.25g,MgSO4.7H2O 0.5g,FeSO4.7H2O 0.001g,Distilled water[蒸餾水] 1000毫升,pH 3.5-4
       
      43. Plasma Substitute Medium[代血漿培養基]
      Sucrose[蔗糖] 12.5-15%,Peptone[蛋白胨] 0.5%,KH2PO4 0.03%,Na2HPO4.12H2O 0.14%,pH 7.0-7.2
       
      44. Skim Milk Medium[脫脂牛奶培養基] ATCC 183
      脫脂牛奶 100g,去離子水 1000毫升
      適用范圍:保加利亞乳桿菌、嗜熱乳酸鏈球菌。
       
      45. Pea Agar[豌豆瓊脂]
      Glucose[葡萄糖] 1%,Peptone[蛋白胨] 0.5%,NaCl 0.5%,Agar[瓊脂] 2.0%,Pea infusion[counted by NH2] [豌豆浸汁[以NH2計]] 12-14 mg∕100,pH[before sterilization] [消前] 7.2-7.5
      [Note]: Preparetion of pea infusion[豌豆浸汁的制法] :
      Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30毫升 of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition .[稱取白豌豆900克,置于5公升廣口瓶中,加自來水3公升,用硫酸調pH至4.3-4.5,再加氯仿30毫升[防腐],加膠塞并用紗布扎口[防止沖脫],放37℃恒溫室7-10天。取出用濾紙過濾,濾液煮沸20分鐘,以除氯仿,并再次以濾紙過濾,除沉淀。濾液分裝,于120℃30分鐘滅菌。后冷藏備用。]
      The standard quality of pea infusion as following[豌豆浸汁質量標準]:
      Aminonitrogen[氨基酸]: 160-200 mg∕100毫升,Soluble phosphoruse[溶磷]: 400-600 μg∕毫升,Color[色澤]: auratus,transparent [金黃、透明]
       
      46. Wheat Bran Medium[麥麩瓊脂]
      Wheat bran[麥麩] 3.6%,[NH4]2HPO4 0.3%,K2HPO4 0.02%,MgSO4.7H2O 0.01%,Agar[瓊脂] 2%,pH 7.0
      適用范圍:金霉素鏈霉菌、紅霉素鏈霉菌。
       
      47. Glucose Yeast Extract Agar[葡萄糖、酵母膏瓊脂]
      Glucose[葡萄糖] 10g,Yeast extract[酵母膏] 10g,Water[水] 1000毫升,Agar[瓊脂] 15g,pH 7.2
       
      48. MMN Medium[MMN培養基]
      CaCl2 0.05g,MgSO4 0.15g,NaCl 0.025g FeCl3[1%] 1.2毫升,KH2PO4 0.5g,Vitamib B1[硫胺素] 100μg,[NH4]2HPO4 0.25g,Wort[12 Be′] 100毫升,Glucose[葡萄糖] 10-15g,Citric acid[檸檬酸] 0.2g,Distilled water[蒸餾水] 900毫升,pH 5.5[左右]
      適用范圍:蜜環菌。
       
      49. Starch Agar[淀粉瓊脂]
      Soluble starch[可溶性淀粉] 10g,NaNO3 1g,MgCO3 1g,K2HPO4 0.3g,NaCl 0.5g,Agar[瓊脂] 20g,Distilled water[蒸餾水] 1000毫升
       
      50. 5 Be′Wort Agar[5 Be′麥芽汁瓊脂]
      適用范圍:園弧青霉、少根根霉、日本根霉、爪哇根霉
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