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      麥康凱瓊脂培養基的配方

      發布時間:

      2022-12-27

      作者:

      青島日水生物


      麥康凱瓊脂培養基配制方法:稱取本品50.0g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。

       
      MacConkey Agar Formula[配方]: g/L
      Gelatin of trypsin digestion content[明膠胰酶水解物] :17.0g
      Peptone[胨]:3.0g                           
      Lactose[乳糖]:10.0g                          
      Sodium Chloride[氯化鈉]:5.0g                     
      Sodium deoxycholate[脫氧膽酸鈉]:1.5g                          
      Neutral Red[中性紅]:0.03g
      Crystal Violet[結晶紫:0.001g                
      Agar[瓊脂]:13.5g                           
      pH7.1±0.2[25℃]
       
      The preparation method of McConkey agar medium: 50.0g of this product in 1L distilled water or deionized water, heated and boiled until completely dissolved, packed, high-pressure sterilization at 121 ~C for 15 minutes, shake well after sterilization, in order to prevent agar from depositing on the bottom of the vessel and solidifying, reserve.
      MacConkey Agar Formula[formula]:g/L
      Gelatin of trypsin digestion content[gelatin trypsin hydrolysate]:17.0g
      Peptone[peptone]:3.0g
      Lactose[lactose]:10.0g
      Sodium Chloride[NaCl]:5.0g
      Sodium deoxycholate[sodium deoxycholate]:1.5g
      Neutral Red[neutral red]:0.03g
      Crystal Violet[crystal violet: 0.001g
      Agar[agar]:13.5g
      PH7.1 + 0.2[25 C]
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