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      腸道菌增菌肉湯(EE肉湯)的配制方法

      發布時間:

      2022-12-27

      作者:

      日水生物


      腸道菌增菌肉湯配制方法: 稱取本品45.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),沸水浴煮沸30分鐘,冷卻至常溫備用。
       
      Formula/配方): g/L
      Tryptone/胰蛋白胨:10.0g
      Dextrose /葡萄糖:5.0g
      Disodium Hydrogen Phosphate
      /磷酸氫二鈉:8.0g
      Monopotassium phosphate
      /磷酸二氫鉀:2.0g
      Bile Salts/牛膽鹽:20.0g
      Brilliant Green/煌綠:0.015g
      pH7.2±0.2 /25℃)
      The preparation method of intestinal microflora broth (EE broth): called this product 45.0g in 1L distilled water or deionized water (can increase or reduce the amount of preparation according to proportion), boiling water bath boiling for 30 minutes, cooling to the room temperature reserve.
      Formula: g/L
      Tryptone: 10.0g
      Glucose: 5.0g
      Two sodium hydrogen phosphate: 8.0g
      Potassium dihydrogen phosphate: 2.0g
      Bovine bile salt: 20.0g
      Brilliant green: 0.015g
      PH7.2 + 0.2 /25 C
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