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      改良頭孢哌酮麥康凱瓊脂(CMA)基礎的配方

      發布時間:

      2022-12-27

      作者:

      日水生物


      改良頭孢哌酮麥康凱瓊脂(CMA)基礎配制方法: 稱取本品67.0g于1L蒸餾水或去離子水中/可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,115℃高壓滅菌15分鐘,冷卻至50-55℃,每100mL培養基中加入1支20222/頭孢哌酮溶液,1.6mg/支),搖勻,備用。
      Formula/配方): g/L 
      Peptone /蛋白胨:17.0g
      Peptone/胨:3.0g
      Bile Salts/牛膽鹽:5.0g
      Sodium Chloride/氯化鈉:5.0g
      Lactose/乳糖:10.0g
      Glucose/葡萄糖:10.0g
      Crystal Violet/結晶紫:0.001g
      Neutral Red/中性紅:0.025g
      Agar/瓊脂:17.0g
      pH7.2±0.2/25℃)
      Preparation method: This product is called 67.0g in 1L distilled water or deionized water / can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 115 centigrade for 15 minutes, cool to 50-55 C, add 1 20222/ cefoperazone solution per 100mL medium, 1.6mg/ branch, shake up and spare.
      Formula: g/L
      Peptone: 17.0g
      Peptone: 3.0g
      Bovine bile salt: 5.0g
      Sodium chloride: 5.0g
      Lactose: 10.0g
      Glucose: 10.0g
      Crystal violet: 0.001g
      Neutral red: 0.025g
      Agar: 17.0g
      PH7.2 + 0.2/25 C)
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