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      改良布氏肉湯培養基的配方

      發布時間:

      2022-12-27

      作者:

      日水生物


      改良布氏肉湯培養基的配方配制方法:
      稱取本品28.6g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,116℃高壓滅菌20鐘,備用。
       
      Formula(改良布氏肉湯培養基配方): g/L
      Tryptone (胰蛋白胨)                        10.0g
      Peptone(蛋白胨)                                    10.0g
      Dextrose(葡萄糖)                                     1.0g
      Yeast Extract(酵母浸膏)                        1.0g
      Sodium Chloride(氯化鈉)                       5.0g
      Sodium Thioglycollate
      (硫乙醇酸鈉)                                            1.5g
      Sodium metabisulfite
      (偏重亞硫酸鈉)                                       0.1g
       pH7.3±0.2(25℃)
       
       
       

      Preparation method:
      Weigh 28.6g of this product in 1L distilled water or deionized water
      Medium (can increase or decrease proportionally), heat and boil
      Until completely dissolved, repackaging, 116 ℃ high pressure sterilization for 20 minutes, and set aside.
       
      Formula: g/L
      Tryptone (Tryptone) 10.0g
      Peptone(Peptone) 10.0g
      Dextrose(glucose) 1.0g
      Calcium Extract(Yeast Extract) 1.0g
      Sodium Chloride (nacl) 5.0 g
      Sodium Thioglycollate
      (sodium thioethanolate) 1.5g
      Sodium metabisulfite
      (partial sodium sulfite) 0.1g
      PH7.3 + / - 0.2 (25 ℃)
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