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      改良克氏雙糖鐵培養基配制方法及使用

      發布時間:

      2022-12-27

      作者:

      日水生物


      改良克氏雙糖鐵培養基配制方法:
      稱取本品65.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝試管,121℃高壓滅菌15分鐘,滅菌結束后制成高層斜面,備用。
       
      Formula(改良克氏雙糖鐵培養基配方): g/L
      Peptone (蛋白胨)                                  20.0g                          
      Beef Extract(牛肉浸出粉)                     3.0g
      Yeast Extract(酵母浸粉)                       3.0g               
      Sodium Chloride(氯化鈉)                           5.0g
      Sorbitol(山梨醇)                                   20.0g
      Dextrose(葡萄糖)                                   1.0g
      Ferric Ammonium Citrate 
      (檸檬酸鐵銨)                                           0.5g
      Sodium Thiosulfate(硫代硫酸鈉)         0.5g
      Phenol Red(酚紅)                              0.025g      
      Agar(瓊脂)                                            12.0g                              
      pH7.4±0.2(25℃)       
       
       

       
      Preparation method:
      Weigh 65.0g of this product in 1L distilled water or deionized water
      Medium (can increase or decrease proportionally), heat and cook
      Boiling until completely dissolved, repackaging tube, 121 ℃ high pressure sterilization for 15 minutes, after the sterilization cant made top, set aside.
       
      Formula: g/L
      Peptone (Peptone) 20.0g
      Beef Extract 3.0g
      Calcium Extract (Yeast Extract) 3.0g
      Sodium Chloride (nacl) 5.0 g
      Sorbitol (Sorbitol) 20.0g
      Dextrose (glucose) 1.0g
      Ferric Ammonium Citrate
      (ammonium ferric citrate) 0.5g
      Sodium Thiosulfate (Sodium Thiosulfate) was 0.5g
      Phenol Red (Phenol Red) 0.025g
      Agar (Agar) 12.0g
      PH7.4 slightly + / - 0.2 (25 ℃)
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