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      麥康凱瓊脂培養基的配制方法

      發布時間:

      2022-12-27

      作者:


      麥康凱瓊脂培養基

       

      英文名稱:MacConkey  Agar
      產品編號:12721-1
      產品規格:250g/瓶
      產品用途:用于大腸埃希氏菌的選擇性分離
       
      Formula(配方): g/L
      Gelatin of trypsin digestion content(明膠胰酶水解物)    17.0g
      Peptone(胨)               3.0g                          
      Lactose(乳糖)             10.0g                         
      Sodium Chloride(氯化鈉)        5.0g                    
      Sodium deoxycholate(脫氧膽酸鈉)      1.5g                         
      Neutral Red(中性紅)      0.03g
      Crystal Violet(結晶紫)    0.001g               
      Agar(瓊脂)                   13.5g                          
      pH7.1±0.2(25℃)
       
      日水培養基的配制方法:
          稱取本品50.0g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝,121℃高
      壓滅菌15分鐘,滅菌結束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。

       


       

      McConkey AGAR medium

       
       
       
      MacConkey Agar
      Product number: 12721-1
      Product specification: 250g/ bottle
      Application: for selective isolation of escherichia coli
       
      Formula: g/L
      Gelatin of trypsin digestion content (Gelatin hydrolysate pancreatic enzyme) 17.0 g
      Peptone (Peptone) 3.0g
      Lactose (Lactose) 10.0g
      Sodium Chloride (nacl) 5.0 g
      Sodium deoxycholate is 1.5g
      Neutral Red is 0.03g Neutral
      Crystal Violet 0.001g
      Agar (Agar) 13.5g
      PH7.1 + / - 0.2 (25 ℃)
       
      Preparation method:
      According to take this product 50.0 g in 1 l of distilled water or deionized water, heating boiling until completely dissolved, repackaging, 121 ℃ high
      Press sterilization for 15 minutes, shake well after sterilization, to prevent agar-agar deposition at the bottom of the container and solidify, set aside.
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