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      胰酪大豆胨瓊脂培養基(TSA)的配制方法

      發布時間:

      2022-12-27

      作者:


      胰酪大豆胨瓊脂培養基(TSA)

       

      英文名稱:Trypticase Soy Agar Medium
      產品編號:12724-1
      產品規格:250g/瓶
      產品用途:用于菌落總數測定、菌種純化、傳代
       
      Formula(配方): g/L
      Pancreatic Digest of  Casein (胰酪胨)          15.0g                        
      Sodium Chloride(氯化鈉)          5.0g              
      Enzymatic Digest of Soybean Meal(大豆木瓜蛋白酶水解物)    5.0g                                                  
      Agar(瓊脂)                  15.0g                      
      pH7.3±0.2(25℃)  
       
      培養基的配制方法:
         稱取本品40.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱攪拌煮沸至完全溶解,分裝121℃高壓滅菌15分鐘,滅菌結束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用
      e:10.5?_x0005_b_x0016_t_x0010_ _x0003_??_x0017_??_x0001_ kerning:1.0000pt; " >1L蒸餾水或去離子水中(可按比例增加或減少配制量),微溫溶解,分裝,121℃高壓滅菌15分鐘,備用。

       


      Trypticase Soy Agar Medium
      Product number: 12724-1
      Product specification: 250g/ bottle
      Product USES: used for the determination of total number of colonies, purification of strains, and propagation
       
      Formula: g/L
      Pancreatic Digest of Casein (Pancreatic Casein) 15.0g
      Sodium Chloride (nacl) 5.0 g
      Papain hydrolysis Enzymatic Digest of Soybean Meal (Soybean) 5.0 g
      Agar (Agar) 15.0g
      PH7.3 + / - 0.2 (25 ℃)
       
      Preparation method:
      According to take this product 40.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling stir until completely dissolved, repackaging 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside
      E: 10.5 ? bt ? ? ? ? kerning: 1.0000 pt; "> 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), dissolve the lukewarm, repackaging, 121 ℃ high pressure sterilization for 15 minutes. Set aside.
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