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      腸道菌增菌液體培養基的制備和配制

      發布時間:

      2022-12-27

      作者:


      腸道菌增菌液體培養基

       英文名稱:Enterobacteria  Enrichment  Broth 

      產品編號:12722-1

      產品規格:250g/瓶

      產品用途:用于耐膽鹽革蘭陰性菌的選擇性增菌

       

      Formula(配方): g/L

      Pancreatic Digest of Gelatin(明膠胰酶水解物)     10.0g

      Dextrose (葡萄糖)           5.0g

      Disodium Hydrogen Phosphate•2H2O (二水合磷酸氫二鈉)     8.0g

      Monopotassium phosphate(磷酸二氫鉀)          2.0g

      Bile Salts(牛膽鹽)             20.0g

      Brilliant Green (亮綠)     0.015g

      pH7.2±0.2 (25℃)

       

      培養基的配制方法:

          稱取本品45.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),沸水浴煮沸30分鐘,冷卻至常溫備用。

       


       

      Intestinal bacterial growth liquid medium

       
       
       
      Enterobacteria Enrichment Broth
       
      Product number: 12722-1
       
      Product specification: 250g/ bottle
       
      Application: it is used for selective enrichment of gallbladder salt gram-negative bacteria
       
       
       
      Formula: g/L
       
      Pancreatic Digest of sunlight 10.0g
       
      Dextrose (glucose) 5.0g
       
      Disodium Hydrogen Phosphate, 2 h2o (Disodium Hydrogen Phosphate hydrate) 8.0 g
       
      Monopotassium phosphate (KDP) 2.0 g
       
      Bile Salts(Bile Salts) 20.0g
       
      Brilliant Green is 0.015 grams
       
      PH7.2 + / - 0.2 (25 ℃)
       
       
       
      Preparation method:
       
      Weigh 45.0g of this product in 1L distilled water or deionized water (can increase or decrease the amount of preparation in proportion), boil in boiling water for 30 minutes, cool to room temperature and set aside.
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