前面幾點講了倒平皿的最適溫度保持在35-40度，但不同品牌的瓊脂質量不一樣，同樣按照國標中的配方瓊脂含量1.5%， 有些瓊脂凝固很快，有些凝固慢一些，還有環境溫度偏低，瓊脂凝固也會快一些，要根據實際情況，適當調整瓊脂的含量，在室溫較低時我們所配的培養基瓊脂 用量就偏少一些，還有在倒平板和混勻時動作要快一些，室溫實在太低，也可以把培養皿放到烘箱中適當加熱一下（40度左右）。
Skills and Notices of Inverting Plate in Laboratory Experiment
In biological experiments, we believe that we often have inverted plates, bacterial culture, molecular biology experiments and so on. In medical enterprises, the inverted plate is more common, environmental monitoring, water monitoring, microbiological inspection and so on, the beginners are often not good, now summarize a few small skills for everyone to refer to:
1. To prevent the condensation of Petri dishes:
1, as is known to all, the palm is not hot, and it is an empirical data. At this time, the temperature is about 45 degrees.
2. If you want to pour a lot of dishes continuously, especially in the winter, it tends to solidify easily. Once it's solidified, it needs to be reheated to a higher temperature to dissolve (the characteristics of the vitreous body). Therefore, it is suggested that a 50 degree constant temperature water bath should be opened at this time, and the effect of putting the triangle bottle into the culture medium at any time will be good.
Two. There is condensate in the culture dish:
Generally speaking, there will be several drops and little influence. If you have too much, you need to pay attention to it. The general situation is:
1, the pan should be kept at a temperature of 35-40 degrees (tentacles with warm feeling) before pouring.
2. Keep the environment at a high temperature.
Three. The agar medium that has been cooled down:
It is a headache for the cooled medium to melt again, because it needs not only patience on the electric stove, but also with care. It will make a wet bottle stopper without mind, and reheat it in a sterilizer. It's good, but it's hard to bother. The solution is to use microwave oven heating, fast and good, melt thoroughly, with ease. Of course, the triangular bottle used in the culture medium can be put into the microwave oven: 250ML.
What do you do without a microwave oven? Be careful with the electric furnace. When melting the nutrient agar directly on the electric furnace, attention should be paid to the use of small fire when melting to the last thin layer above, slowly heating, preventing the gas from breaking the thin layer and causing the boiling, and nutrient agar out of the trigonometric bottle.
Four. The Petri dish remains homogeneous.
Pour the pan into the left three circle, shake the right three circles. Be careful not to dip the pan cover and shake gently.
Five, specific treatment:
The first few points said that the optimum temperature of the plate is kept at 35-40 degrees, but the quality of the different brands of agar is not the same, as well as the content of the formula agar in the national standard 1.5%, some agar solidified quickly, some solidified slowly, and the environment temperature is low, agar will solidify faster, according to the actual situation, the appropriate adjustment The agar content of the agar at room temperature is less than that of the medium agar at room temperature. It also moves faster when the plate is poured and mixed, it is too low at room temperature and can be properly heated in a oven (about 40 degrees).
Six. Avoid bubbles:
Students are reflected in the inverted plate will produce bubbles, mainly comply with the above points, the heating should not be too high, melting can, strengthen the operation practice, and it is the time to slow down, it is best to stick to the wall of the plate down.